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Image Search Results
Journal: iScience
Article Title: A non-invasive method to genotype cephalopod sex by quantitative PCR
doi: 10.1016/j.isci.2026.115594
Figure Lengend Snippet: Overview of the sex genotyping protocol Coleoid cephalopods, including dwarf cuttlefish ( Ascarosepion bandense ), California two-spot octopus ( Octopus bimaculoides ), common cuttlefish ( Sepia officinalis ), hummingbird bobtail squid ( Euprymna berryi ), longfin inshore squid ( Doryteuthis pealeii ), northern shortfin squid ( Illex illecebrosus ), and Argentine shortfin squid ( Illex argentinus ), appear to have a ZZ/Z0 sex determination system, in which males carry two copies of the Z chromosome and females carry one. Sex is determined by measuring the abundance of a Z-linked sequence relative to an autosomal sequence using qPCR after genomic DNA extraction from a skin swab or tissue sample
Article Snippet:
Techniques: Northern Blot, Sequencing, DNA Extraction
Journal: iScience
Article Title: A non-invasive method to genotype cephalopod sex by quantitative PCR
doi: 10.1016/j.isci.2026.115594
Figure Lengend Snippet: Conserved synteny and homology reveal orthologous sex chromosomes in multiple coleoid species Riparian synteny plot of the Z chromosomes of O . bimaculoides , E . scolopes , E . berryi , D . pealeii , S . officinalis , and S . esculenta , with E . scolopes set as the reference species. Z chromosomes from S . officinalis , D . pealeii , and O . bimaculoides (marked with asterisks) were inverted to improve visualization. See also .
Article Snippet:
Techniques:
Journal: iScience
Article Title: A non-invasive method to genotype cephalopod sex by quantitative PCR
doi: 10.1016/j.isci.2026.115594
Figure Lengend Snippet: Quantitative PCR shows sex-specific dosage differences in seven octopus, cuttlefish, and squid species (A) A . bandense . (B) D . pealeii . (C) E . berryi . (D) O . bimaculoides . (E) S . officinalis . (F) I . illecebrosus . (G) I . argentinus . For each species, ΔΔC q values were calculated between autosomal and sex-linked loci across eight blinded, randomized tissue samples. Values were normalized such that the median of the higher-ΔΔC q cluster (male) was set to zero. Each datapoint represents the mean value of four technical replicates from a single animal ( N = 8 animals for each species). Datapoints are colored by post-mortem-confirmed sex (females, red; males, blue). Box-and-whisker plots show the median, interquartile range, and whiskers extending to the most extreme non-outlier values. ∗∗∗∗ p < 1 × 10 −4 (Student’s t test). See also .
Article Snippet:
Techniques: Real-time Polymerase Chain Reaction, Whisker Assay